The extraction of folic (pteroylglutamic) acid conjugate from yeast.
نویسنده
چکیده
The conjugate of folic acid (pteroylglutamic acid, vitamin B,) in yeast was isolated by Pfiffner et al. (1) and determined to be pteroylhexaglutamyl glutamate (2). Recently this group has published details of the concentration of the conjugate (3). The present paper describes a simple method for producing a concentration of the conjugate of from 40to loo-fold by differential extraction of columns of yeast with alcohol-water mixtures. Further purification was attained by fractional precipitations in 70 per cent alcohol at different pH levels.
منابع مشابه
The comparative utilization of pteroylglutamic acid and pteroyltriglutamic acid by chicks on a purified diet.
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 179 3 شماره
صفحات -
تاریخ انتشار 1949